AGFusion

Overview

AGFusion (Annotate Gene Fusion) is a Python package for annotating and visualizing gene fusions. Given a fusion event (gene A and gene B with breakpoint coordinates), AGFusion predicts the protein domain structure of the resulting chimeric protein by mapping Pfam domain annotations (from UniProt/PFAM) onto the fusion junction. This allows automated determination of whether key functional domains (e.g., kinase domains) are retained or disrupted in the fusion protein.

Used by

• Used to infer kinase-domain integrity for 2,892 kinase-partner fusions across 9,624 TCGA tumor samples; determined that 1,275 (44.1%) of kinase fusions retain an intact kinase domain, a key criterion for identifying potentially activating fusion events PMID:29617662

Notes

• Annotations are based on UniProt/PFAM domain databases; results depend on database version.
• Domain-retention status is predictive but not equivalent to functional validation of kinase activity.
• Enables automated classification of 3′-kinase vs. 5′-kinase fusions for mechanistic interpretation.

Sources

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