DynaMut

Overview

DynaMut is a web server and computational tool for predicting the effect of amino acid substitutions on protein stability using normal mode analysis (NMA) and graph-based signatures derived from atomic interactions. It takes a PDB structure file and the variant of interest, then computes the change in free energy (ΔΔG) upon mutation. Positive ΔΔG values indicate predicted protein stabilization; negative values indicate destabilization. DynaMut2 is an updated version with improved accuracy.

Used by

• Applied to predict the structural effect of UBE2A p.(Arg6Trp) in endometrial polyps using PDB structure 6CYO; computed ΔΔG = +1.650 kcal/mol, predicting a stabilizing effect consistent with a potential gain-of-function mechanism PMID:28445112

Notes

• Requires a 3D protein structure (PDB entry or homology model) as input.
• Positive ΔΔG = stabilizing; negative ΔΔG = destabilizing (note: sign convention differs from some other tools — always check the tool’s definition).
• Used in concert with sequence-based scores (CADD, REVEL, AlphaMissense) for comprehensive variant interpretation.

Sources

• PMID:28445112

This page was processed by crosslinker on 2026-05-14.