Illumina Infinium HumanMethylation27 BeadChip (HM27)

Overview

The Illumina Infinium HumanMethylation27 BeadChip (HM27) is a genome-wide DNA methylation array covering approximately 27,000 CpG loci. It quantifies bisulfite-converted DNA using two-color fluorescent detection, reporting beta values (0–1) for each probe. The HM27 platform was widely used in early TCGA studies for epigenomic profiling, and has since been superseded by the higher-density HM450 array.

Used by

• Applied to 283 GBM samples (plus 91 reanalyzed) as part of TCGA GBM 2013 multi-platform methylation analysis; contributed to the identification of G-CIMP methylation subclasses and the six-class methylation model (M1–M6) PMID:24120142
• Included as one of the methylation platforms in the TCGA ILC/IDC multi-platform profiling (n=817 tumors); methylation data contributed to epigenetic characterization showing no CDH1 promoter hypermethylation in ILC PMID:26451490
• Used for DNA methylation profiling of 287 GBM samples in the TCGA pan-glioma study; merged with HM450 data on 1,300 tumor-specific CpG probes to define six pan-glioma methylation clusters (LGm1–6) PMID:26824661

Notes

• The HM27 covers ~27,000 CpG sites, primarily in promoter regions; it is less comprehensive than the HM450 array (~450,000 CpG sites).
• G-CIMP classification derived in part from HM27 data defined IDH1-mutant GBM epigenotype with favorable prognosis.
• Being superseded by HM450 and EPIC arrays in newer studies.

Sources

This page was processed by crosslinker on 2026-05-14. - PMID:26451490

This page was processed by crosslinker on 2026-05-14. - PMID:26824661

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