Intramuscular Xenograft

Overview

In vivo tumor model in which human cells are injected directly into muscle tissue (typically gastrocnemius) of immunocompromised mice; used to model soft-tissue sarcomas or assess tumorigenic potential of cell populations in a physiologically relevant anatomical site.

Used by

• EWS::FLI1-transduced heMSCs (1×10^6 cells in Matrigel) injected intramuscularly into the gastrocnemius of 21-day-old NOD/SCID mice; ~40% tumor penetrance (~5-month latency) produced soft whitish spinal masses, hemorrhagic abdominal/mesothelial masses, and lung/liver/kidney metastases with classic Ewing sarcoma histology (small round blue cells). PMID:25186949

Notes

• Intramuscular route is preferred over subcutaneous for Ewing sarcoma models to better recapitulate the anatomical context of primary bone/soft-tissue tumors.
• Tumors were positive for human HLA, Ku80, Flag (EWS::FLI1), PRKCB, BRCA1, Ki67, and cyclin A by IHC.

Sources

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