Karyotyping

Overview

Karyotyping is a cytogenetic technique that visualizes the chromosomal complement of a cell by staining metaphase chromosomes (classically with G-banding) and arranging them in a standard order for counting and structural analysis. It provides a direct, low-resolution assessment of chromosome number, gross structural rearrangements (translocations, inversions, deletions), and gains/losses at the whole-chromosome or arm level.

Used by

• Used to confirm arm-level hemizygosity of chr_3p in CRISPR-Cas9-engineered AALE cell clones, alongside Sanger sequencing, qPCR, and whole-genome sequencing; provided orthogonal cytogenetic verification of the chromosome arm deletion PMID:29622463

Notes

• Resolution is limited to ~5–10 Mb; focal copy-number changes or small indels require molecular methods (qPCR, sequencing).
• Provides direct visualization but is labor-intensive and requires actively dividing cells in metaphase.
• Complementary to molecular methods (WGS, qPCR, FISH) for confirming engineered chromosomal rearrangements.

Sources

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