Multiplexed immunofluorescence

Overview

Multiplexed immunofluorescence (mIF) enables simultaneous detection of multiple protein markers in tissue sections using spectrally resolved fluorescent antibodies. The Vectra platform supports up to 7+ fluorescent channels for spatial immune profiling of the tumor microenvironment PMID:38653864.

Used by

PMID:38653864 — Vectra 7-color multiplexed immunofluorescence microscopy was applied to 25 of 35 patients with dMMR/MSI-H/hypermutated gynecologic cancers on a nivolumab phase 2 trial; 7-marker panel (CD8, PD-1, TOX, PD-L1, PAX8, FoxP3, DAPI) used to characterize the tumor immune microenvironment and identify predictors of response PMID:38653864.
PMID:39386723 — CyCIF (cyclic immunofluorescence) with a 31-antibody panel applied to 44 fallopian tube FFPE specimens spanning the HGSOC progression axis (p53 signatures through invasive cancer); 3D CyCIF on a Zeiss LSM980 confocal used for one STIC.C case to validate micronuclear rupture and cGAS recruitment; quantification by MCMICRO Nextflow + UNMICST2 segmentation pipeline; identified stepwise increase in HLA-E+ and p-STAT1+ epithelial cells and NK–cDC1 axis collapse from STIC.I to STIC.C PMID:39386723.
Applied to 100 HGSOC multi-site biopsy samples; showed CD8+PD-1+ T cells within 30 um of PD-L1+ cancer cells common in HRD-Dup but rare/absent in FBI tumours; confirmed spatial immune-exclusion pattern in FBI PMID:36517593
Multiplexed immunofluorescence (MxIF) performed on 34 WU-RCC patients with a 19-marker ccRCC antibody panel for spatial proteomics validation of HiTME subtypes PMID:22138691

Notes

Multiplexed IF revealed spatial relationships between CD8+ T cell exhaustion markers (TOX, PD-1) and tumor cells (PAX8) in the context of nivolumab response PMID:38653864.
Corpus-grown slug; not present in canonical ontology.

Sources

This page was processed by crosslinker on 2026-05-05. - PMID:36517593

This page was processed by crosslinker on 2026-05-05. - PMID:22138691

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