Prostate Organoid (Pten/Lkb1 Murine Model)

Overview

This model uses prostate cancer organoids derived from Pten^pc-/-;Lkb1^pc-/- (prostate-specific Pten and Stk11/Lkb1 double-knockout) C57BL/6J mice. The compound knockout generates murine prostate tumors with aggressive features, and primary cells can be cultured as three-dimensional organoids to assess the functional contribution of candidate cancer genes to proliferation, organoid formation, and growth. Gene knockdown or overexpression is introduced via lentiviral delivery of shRNA or sgRNA, and organoid number and size are quantified as readouts.

Used by

• NOL10 and USF1 shRNA/sgRNA knockdown performed in Pten^pc-/-;Lkb1^pc-/- prostate cancer organoids; NOL10 and USF1 loss produced significantly fewer and smaller organoids, confirming the oncogenic role of the rs4519489–USF1–NOL10 axis in a genetically engineered murine prostate cancer model PMID:28927585

Notes

• Provides an in vivo-derived, immunocompetent background for organoid experiments, complementing xenograft and cell-line studies.
• LKB1 corresponds to the STK11 gene; in this model it is co-deleted with PTEN to model aggressive prostate cancer.
• Corpus-grown slug reflecting the specific genotype (Pten^pc-/-;Lkb1^pc-/-) used in this study.

Sources

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