Reduced representation bisulfite sequencing (RRBS)

Overview

Restriction-enzyme-based bisulfite sequencing approach that enriches CpG-dense regions for cost-effective DNA methylation profiling.

Used by

• PMID:35927489 — RRBS performed on n=509 CLL samples (part of the n=999 methylation cohort, complementing 450k array n=490) and used to extend the n-CLL/i-CLL/m-CLL epitype framework and the epiCMIT mitotic clock PMID:35927489.
• eRRBS (enhanced Reduced Representation Bisulfite Sequencing) used for genome-wide single-cytosine methylation profiling of 114 metastatic CRPC biopsies; strong epigenetic segregation of CRPC-NE from CRPC-Adeno identified SPDEF promoter hypermethylation and re-classified 3 histologic adenocarcinomas as CRPC-NE PMID:26855148

Notes

• DNA methylome variation in CLL was dominated by cell-of-origin and proliferative history rather than specific driver mutations PMID:35927489.

Sources

• PMID:35927489

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