Single-nuclear RNA sequencing (snRNA-seq)

Overview

Single-nuclear RNA sequencing (snRNA-seq) is a single-cell transcriptomics method that works on nuclei isolated from frozen tissue, enabling cell type decomposition and transcriptional characterization in samples where intact cells cannot be recovered PMID:34433969.

Used by

• PMID:34433969 — droplet-based snRNA-seq was performed on 8 meningioma tumors and 2 healthy meninges (54,393 nuclei total) from the University Health Network Brain Tumor BioBank; identified cell-type-specific transcriptional programs distinguishing meningioma molecular groups (MG1–MG4) and characterized the meningioma tumor microenvironment PMID:34433969.
• PMID:34493726 — Smart-Seq2-based snRNA-seq (nuc-Seq) on 11 neuroblastoma tumors (4,224 nuclei; 3,212 high-quality) and 3 postnatal human adrenal glands (1,536 nuclei; 1,322 high-quality) across INRG risk groups; identified undifferentiated nC3 cluster in high-risk disease resembling a novel postnatal human cholinergic progenitor PMID:34493726.

Notes

• snRNA-seq data contributed to functional interpretation of the COCA-derived molecular groups in meningioma PMID:34433969.
• Corpus-grown slug; not present in canonical ontology.

Sources

• PMID:34433969
• PMID:34493726

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