CyCIF (Cyclic Immunofluorescence)

Overview

CyCIF is a multiplexed cyclic immunofluorescence imaging method that enables detection of dozens of protein markers in situ using iterative rounds of staining, imaging, and signal bleaching on FFPE tissue sections PMID:39386723.

Used by

• PMID:39386723 — CyCIF with a 31-antibody panel was applied to 44 fallopian tube specimens (including HGSOC precursors: normal FTE, SCOUT, STIC, invasive HGSOC) to characterize the tumor immune microenvironment; revealed progressive immune suppression from FTE through STIC to invasive HGSOC, including increased PD-L1 expression and reduced CD8+ T cell infiltration PMID:39386723.

Notes

• Combined with GeoMx WTA spatial transcriptomics to provide complementary protein and transcriptomic spatial profiling of HGSOC precursor lesions PMID:39386723.
• Corpus-grown slug; not present in canonical ontology.

Sources

• PMID:39386723

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